{"id":3112,"date":"2023-05-17T09:08:06","date_gmt":"2023-05-17T07:08:06","guid":{"rendered":"https:\/\/www.co2news.sk\/?p=3112"},"modified":"2023-05-17T09:08:06","modified_gmt":"2023-05-17T07:08:06","slug":"converts-air-captured-co2-with-methanol-into-amino-acid-and-pyruvate-in-a-chemoenzymatic-system-without-atp-naph","status":"publish","type":"post","link":"https:\/\/www.co2news.sk\/en\/2023\/05\/17\/converts-air-captured-co2-with-methanol-into-amino-acid-and-pyruvate-in-a-chemoenzymatic-system-without-atp-naph\/","title":{"rendered":"Convert air-entrained CO2 with methanol to amino acid and pyruvate in an ATP\/NAD(P)H-free chemoenzymatic system"},"content":{"rendered":"<p>The use of gaseous and air-captured CO2 for technical biosynthesis is highly desirable, but so far elusive due to several obstacles including high energy consumption (ATP, NADPH), low thermodynamic driving force, and limited biosynthesis rate. Here we present an ATP- and NAD(P)H-free chemoenzymatic system for the biosynthesis of amino acids and pyruvate by combining methanol with CO2. It relies on a redesigned glycine cleavage system with the NAD(P)H-dependent protein L replaced by the biocompatible chemical reduction protein H dithiothreitol. The latter provides a higher thermodynamic driving force, determines the direction of the reaction and prevents protein polymerization of the rate-limiting enzyme carboxylase. Designing the H protein to efficiently release the lipoamide arm from the protected state further enhanced the performance of the system, achieving synthesis of glycine, serine, and pyruvate at g\/lz methanol and air-captured CO2 levels. This work opens the door to the biosynthesis of amino acids and derived products from air. Photosynthesis as a form of biosynthesis using CO<sub>2<\/sub> from the atmosphere and solar energy formed the birth and past of life on Earth. Technical biosynthesis using gaseous and air-derived CO<sub>2<\/sub> will certainly affect the future of our planet and man due to the ever-increasing anthropogenic CO emissions<sub>2<\/sub>, which threaten the balance of the planetary climate. For the sustainable development of human beings, we face other global challenges such as the supply of goods and food for an ever-growing population that requires the use of CO<sub>2 <\/sub>as an abundant source of carbon with a sustainable energy input to replace fossil carbon and even carbohydrates in commodity production.\u00a0 <em>(Jianming Liu, Han Zhang, Yingving Xu, Hao Meng, An-Ping Zeng, Nature Communications)<\/em><\/p>","protected":false},"excerpt":{"rendered":"<p>The use of gaseous and air-captured CO2 for technical biosynthesis is highly desirable but has so far been elusive due to several obstacles including high energy consumption (ATP, NADPH), low thermodynamic driving force, and limited biosynthetic rate. Here we present an ATP- and NAD(P)H-free chemoenzymatic system for the biosynthesis of amino acids and pyruvate by coupling methanol with CO2. It relies on a redesigned cleavage system [\u2026]<\/p>","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3112","post","type-post","status-publish","format-standard","hentry","category-znizovanie_co2_cdr_ccs_ccu_dac"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/posts\/3112","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/comments?post=3112"}],"version-history":[{"count":0,"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/posts\/3112\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/media?parent=3112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/categories?post=3112"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.co2news.sk\/en\/wp-json\/wp\/v2\/tags?post=3112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}